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The Fiber Disease

Human Anatomy, Physiology, and Medicine. Anything human!

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Postby Skytroll » Tue Jan 31, 2006 5:22 am

Here is something very interesting. After looking at where fungi and algae are then check this out.

http://bio.rutgers.edu/euglena/ret_lab/research.htm

The second major aspect of this research is a comparative study of the primary and secondary structure of the small subunit rRNA from euglenoids. Because of its highly conserved nature, the rRNA molecule can be used effectively to infer phylogenetic relationships among organisms ranging from prokaryotes to man. The SSU rRNA gene is amplified, and cycle sequenced. Sequences are generated, aligned based on primary and secondary structure of the rRNA molecule, and compared. The phylogenetic relationships among the taxa are then inferred. Data from the combined ultrastructural and molecular studies are used to deduce evolutionary relationships among taxa, and to determine the morphological and molecular features which characterize these ancient eukaryotes.

A complete introduction to the euglenoid flagellates is presented our NSF sponsored website entitled the The Euglenoid Project.


They are deducing evolutionary relationships.
So, not discovering it, but, deducing it.

Is this good science? Cladistics?

Protists, of which we have, I believe, are being used to link evolution to man. They even say so here.

This goes back to 1841. Will find the link to that. Is in Spainish.

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Postby Skytroll » Tue Jan 31, 2006 5:55 am

Cyanobacteria and other microbes used for cleanup.

However, could cause quorum sensing in soil?

http://www.heartlandmicrobes.com/SoilRemediation.html



BioZyme’s microorganisms primary composition includes Bacillus, Pseudomonas, Azotobacter, Azospirillium, Rizobium and soil borne Cyanobacteria. BioZymes includes aerobic, anaerobic and faculatative anaerobes that are naturally occurring with no genetically engineered organisms and no pathogens. Although the above microbes are the primary composition of BioZymes, there are 7 families approximately 20 genera and over 30 species in BioZymes. BioZymes is a unique blend of indigenous microorganisms capable of breaking down carbon-based hazardous and non-hazardous chemicals, both chain and aromatic in nature. BioZymes has a broad and divers use in bio remediation of chemicals spills. BioZyme eliminates the “dig and haul” method of cleanup in most cases. If the spill is in an area where it is of no danger to humans or animals, it can be remediated on site. BioZyme is capable of remediating a contaminated site down to acceptable levels or below. BioZymes in not a laboratory engineered (specific use) microbial solution. Another area of remediation that BioZyme is of great use is in the breakdown of animal and human waste. Whether it is a septic system, city municipality or an animal waste lagoon, BioZymes will reduce solids, odors and toxins.

Now if two or more are put together, do they create a large biofilm? Then when this dries out
are the microbes changed?

Note the microbes used.....bacillus, Rhizobium etc....cyanobacteria......Put those tog, you got a machine, eating up the fermenting goo. What if the little buggies get in it and spread it around on the plants etc. OHHHHHHHH, how did that happen.

Biofilms everywhere. How do you kill the microbes once you have killed the metal, or oil, or whatever.

BioZymes, all natural, they say.

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Postby London » Tue Jan 31, 2006 9:43 am

Sky,

I think to answer your quetion about what kills what....

I think the worm will strrike then I believe the Bacteria ( remember...more thatn one) will kill off the worm.

In our case: I predict that to be Pseudomonias A.(SP???
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Postby Barz » Tue Jan 31, 2006 1:05 pm

I do not remember if this was ever posted. I found this very, very interesting. PLEASE READ, SKYTROLL,LONDON, RANDY,JILL

http://www.nap.edu/readingroom/books/bmm/#NOTICE

I have not completed the article. See what you think.

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Re: Ok perhaps you have a better way?

Postby givemeabreak » Tue Jan 31, 2006 2:55 pm

southcity wrote:of all the people to be worried about making this illness less credible or us look like idiots, if this is what you feel I have done then gladly I will blend right back into the background and disappear along with the statistic. My whole entire goal is solely to get public attention and to get the uninfected to start asking questions. period. until this begins the scientific community continue to ignore this. I am sure of this, until at least someone can prove me wrong. as to what means gets them asking questions, I truly don't care what it takes. right info, wrong info, who cares as long as this gets in the publics consciousness. because until then we will CONTINUE to be ignored. Curious, Do you doubt this statement as well? If you do then either you are not infected, only infected a short time, or you are an person with another agenda or lastly just plain ignorant. So far as of yet I have seen NO BETTER explanation and no where close to anything else which may gather public interest in this illness. So you see as far as I can tell, in getting the desired goal, it does not matter one bit whether tam is right or wrong, but let me tell you something, the visitors that are coming are everyone you would expect them to be if he is RIGHT..


do not think I am some armchair sitting, beer guzzling moron who lacks the ability to think critically. That would be a mistake. But if you still feel I am doing more harm than good and that is the consensus, then for sure I will shut the site down. I do not wish to damage the chances of valid research beginning. If you have a better plan to get this illness looked into any faster than it is now, then lets hear it. until then give me a break


Like I said. I don't care what you do as long as you make it clear you speak for yourself, and not all sufferers.

Simple concept. Simple request.

You want public awareness. Sounds reasonable. MRF asked for candidates to be interviewed for their next segment (of which there have been many, as well as the NPA). Did you contact them to participate in those interviews? That would also be exposure.

As for the rest of your comments, such as I having some sort of agenda or insinuating you were a beer guzzling moron, that's just your angry rambling and is not germane to the prior concept.
Last edited by givemeabreak on Tue Jan 31, 2006 3:35 pm, edited 1 time in total.
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Postby Barz » Tue Jan 31, 2006 3:09 pm

Regarding my post above, please note the names and companies associated in this bizzare study. EXXON - Yes Exxon who's last Q profits exceeded the GNP of 125 Countries COMBINED!!!!! $10 BILLION DOLLAR PROFIT IN ONE QUATER!!!!!

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Postby Skytroll » Tue Jan 31, 2006 5:33 pm

Barz,

Thanks for the link....

I do believe that this may tell us who is involved and what their plans are.

I see Rummmmm has a new weapon.
C central is losing some folks.

I think what we have is small compared to what really is happening.

And don't even argue with the nincompoops who are dissing all our work.

It will not have gone in vain.

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Postby Skytroll » Tue Jan 31, 2006 5:37 pm

London,

I know the bacillus Thering. is one bacteria that has been used by agriculture, but, I do believe they changed some things and auerg. is also being used.

It is hard to keep up on studies and organisms that are being used.

But, nevetheless, there are so many different ones used for different purposes, but, the end result is to synthesize right down to all living thing's cells, mitochondria, moving parts, etc.

So, the molecular is the level, metabolic, the very juice that makes us work.

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For Mr. Hill

Postby C3 » Tue Jan 31, 2006 6:59 pm

A 34-year-old woman presented in September 1998 with spontaneous pain and swelling of the right index finger at the proximal palmar interphalangeal crease. There was no associated history of trauma, with the exception of a bee sting in the same area that had occurred in 1996. The patient's hobbies included scuba diving and maintaining an aquarium. On examination of her finger, a discrete mass was noted, and a clinical diagnosis of giant cell tumor of the tendon sheath was made. Surgical exploration of the nodule was consistent with an infectious process, and tissue was sent for bacterial, fungal, and mycobacterial cultures, including Mycobacterium marinum and Mycobacterium haemophilum. All cultures were recorded as negative for growth. The patient failed to respond to various antimicrobial therapies, which included azithromycin dihydrate and vancomycin, and the surgical wound did not heal spontaneously. Several subsequent attempts at debridement and surgical closure of the chronically open and draining wound failed, while repeat cultures of the drainage were positive for Streptococcus agalactiae but negative for mycobacteria.

The patient was referred to the Henry Ford Hospital, Detroit, Mich, in early November 1999 for reevaluation. A radiograph of the lesion showed soft tissue swelling with intact bone along the entire index finger. There was no joint space narrowing at the proximal interphalangeal joint or any other joint of the right hand. Ultrasound of the lesion could not rule out the presence of a foreign body, but magnetic resonance imaging highlighted a soft tissue abnormality consistent with granulation tissue. A provisional diagnosis of a foreign body infection with the possibility of a marine or aquarium-associated microorganism was considered. Excision of the flexor tenosynovium was performed.

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The microbiologic testing of the tissue included Gram stain with aerobic and anaerobic bacterial cultures, acidfast bacilli smear, mycobacterial culture, fungal smear, and fungal cultures. Once again, all microbiological results were negative.

Histopathologic examination of the specimen revealed necrotizing granulomatous inflammation of the subcutaneous tissue (Figure, A). The hematoxylin-eosin-stained sections demonstrated spherical organisms of varying size with central basophilia located within the necrotic granuloma (Figure, A).

The organisms were stained with Gomori methenamine silver (Figure, B and D) and mucicarmine (Figure, C) stains, which highlighted the internal septation and double-layer cell wall.

The biopsy revealed predominantly fibrous tissue with prominent chronic inflammation and a focus of necrotizing granulomatous inflammation containing numerous nonbudding cells, ranging in size from 3 to 30 (mu)m in diameter. On hematoxylin-eosin-stained sections, the organisms had a hyaline appearance with variable degrees of central basophilia. Staining with Gomori methenamine silver and periodic acid-Schiff demonstrated internal septation and multiple endospores (2-4 (mu)m). A mucicarmine stain of the tissue was focally positive, but no capsular material was identified surrounding the organisms.

What is your diagnosis?

Pathologic Diagnosis: Subcutaneous Protothecosis

Prototheca species are aerobic, achlorophyllous, algaelike, unicellular organisms. The primary cell or spherule is known as the theca, and the appearance of the autospherulating theca has been termed mulberry-like endosporulation,l morula,2 and frambasiform.3 Prototheca were first recognized as human pathogens in 1964.4 To date, 7 species of Prototheca have been identified, but only 2 have been associated with human disease, namely, Prototheca wicker-- hamii and Prototheca zopfii.

Prototheca species are widely distributed in the environment and have been isolated from water, sewage, and soil, but fewer than 100 cases of human infection have been reported in the literature.5,6 Trauma and contaminated water are the most common vectors of infection,7 while the face and exposed extremities are the usual sites of cutaneous protothecosis. Protothecosis has been reported in both immunocompromised and immunocompetent individuals, and clinical presentations of infection include focal or disseminated, chronic cutaneous/subcutaneous infection,6 bursitis,1 and, rarely, systemic infection.8 Soft tissue infections favor the olecranon bursa, sites of trauma, or surgical wounds exposed to soil or water (eg, hand tendon repair). The cutaneous lesion may vary in appearance, depending on the immune status of the individual. Immunocompromised patients typically present with vesicobullous and ulcerative lesions, purulent discharge, and crusting. In contrast, immunocompetent individuals usually develop lesions that range from slightly erythematous nodules and papules to eczematous plaques with or without associated cellulitis.9 Gradual enlargement of the lesions typically occurs over weeks to months, with no tendency for spontaneous resolution.10

The diagnosis of protothecosis is generally made by direct identification of the organism in histologic sections or by culture of the organism from infected tissue. The cells of P wickerhamii are round in comparison to the oval or cylindrical shapes of P zopfii. Both species reproduce by internal septation, resulting in the formation of sporangia. Each sporangium contains up to 20 endospores that are released on rupture. The sporangia of P wickerhamii (3-13 (mu)m) are smaller than those of P zopfii (14-16 (mu)m). The latter species produces sporangia less frequently. Organisms that morphologically mimic Prototheca include those capable of forming endosporulating spherules, such as Coccidioides immitis, Rhinosporidium seeberi, and Chlorella species. Coccidioides immitis and R seeberi form sporangia 2 to 10 times larger than protothecal sporangia.2 Lesions produced by Chlorella species are reported to be green on gross examination. Moreover, they contain a greater amount of periodic acid-Schiff-positive, diastase-sensitive granules than do Prototheca species, owing to the starch present within the cytoplasm.3

Nonspherulating organisms mimicking Prototheca species include Cryptococcus neoformans, Blastomyces dermatitidis, and Paracoccidioides brasiliensis.2 Cryptococcus neoformans is an encapsulated budding yeast, whereas B dermatitidis produces broad-based budding yeast cells and P brasiliensis forms multiple buds on a single yeast cell.3

In general, protothecosis does not resolve spontaneously.10 The drug of choice for systemic or disseminated protothecosis is Amphotericin B, while the role of imidazole is unclear. Focal cutaneous or subcutaneous infection can be treated with surgical excision with or without Amphotericin B. Our patient was treated surgically without recurrence to date.

References

1. DeMontclos M, Chatte G, Perrin-Fayolle M, et al. Olecranon bursitis due to Prototheca wickerhamii, an algal opportunistic pathogen. Eur J Clin Microbiol Infect Dis. 1995;14:561-562.

2. Chandler FW, Watts JC. Pathologic Diagnosis of Fungal Infections. Chicago, III: ASCP Press; 198:6-7, 45-53, 161-175 .

3. Boyd AS, Langley M, King LE Jr. Cutaneous manifestation of Prototheca infections. J Am Acad Dermatol. 1995;32:758-764.

4. Davies RR, Spencer H, Walkelin PO. A case of human protothecosis. Trans R Soc Trop Med Hyg. 1964;58:448-451.

5. Wirth FA, Passalacqua JA, Koa G. Disseminated cutaneous protothecosis in an immunocompromised host: a case report and literature review. Cutis. 1999; 63:185-188.

6. Walsh SV, Johnson RA, Tahan SR. Protothecosis: an unusual cause of chronic subcutaneous and soft tissue infection. Am J Dermatopathol. 1998;20:379382.

7. Woolrich A, Koestenblatt E, Don P, et al. Cutaneous protothecosis and AIDS. Am Acad Dermatol. 1994;3:920-924.

8. Kunova A, Kollar T, Spanik S, Krcmery V Jr. First report of Prototheca wickerhamii algaemia in an adult leukemic patient [letter]. J Chemother. 1996;8:166167.

9. Tejada E, Parker CM. Cutaneous erythematous nodular lesion in a crab fisherman. Arch Dermatol. 1994;130:244-245, 247-248.

10. Bennett JE. Miscellaneous fungi, and prototheca. In: Mandell CL, Bennett JE, Dolin R, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Disease. 4th ed. New York, NY: Churchill Livingstone Inc; 1995:23892391.

Hina S. Qureshi, MD; W. Michael Dunne, PhD; Raouf E. Nakhleh, MD

Accepted for publication May 23, 2000.

From the Department of Pathology, Henry Ford Hospital, Detroit, Mich.

Reprints not available from the author.

Copyright College of American Pathologists Mar 2001
Provided by ProQuest Information and Learning Company. All rights Reserved
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Postby C3 » Tue Jan 31, 2006 7:43 pm

What You suffer from is a modified cyanobacterial like(CBL) quorum sensing made micro organism.
(as earlier stated)

This micro organism knows various stages
including a diversification in the form of a set
commensal resembling micro organisms.

They resemble most cocci/ coccidioid like micro organisms are with the potential to disperse in tissue.

This modified micro organism (one and a set diversification) has been well recorded but must be screened.

Screening of the series is research budget depending.

Based on its architecture it is not very likely that a simple protocol will clear infection because various algae and a trypanosoma like element seem to be part of its molecular design.

More distinct reject in the form of dominant gene expression has been recorded.

It is likely that a percentage of the population may turn out positive for this pathogenic element.

This element is without any doubt product of
21th century science and may become topic of fierce international debate.


Sincerely,
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Postby C3 » Tue Jan 31, 2006 7:44 pm

errata: and are with the potential etc.
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Postby RANDY » Tue Jan 31, 2006 8:07 pm

BTW: Takes a while till the sound comes up if you have dial-up.
During the End Times, Good will battle Evil. Where do you stand?
http://unknownskindisease.com
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