DNA Conc

[angela.morris]

How do you work out DNA concentration?

I have spec DNA- result 0.023
Dilution 1/1000

Should my answer be 1150ug/ml?

How do i translate this to ng/ul?

Thanks

[adidasty]

Do you have volume or anything else?

[Dott. Berrino]

Hi angela.morris

You are right your concentration is 1150ug/ml

What you read with spectrophotometer is "Absorbance" of your sample
you have to multiply this number for a coeffiicient that is 50 for DNA and for your diluition factor (1000 in your case)
0.023 x 50 x 1000= 1150ug/ml

(This formula is known as Lambert-Beer)

To convert into ng/ul is very simple: is exactly the same 1150ng/ul

Ciao

[Chris4]

Sounds correct. Just to add that the conversion to ng/ul from ug/ml is exactly the same because ng is 10-9, ug 10-6 and ml 10-3. Thats 10 to the power of -9 etc. Therefore 10-6/10-3 is same ratio as 10-9/10-6. Works if you do the maths.
Please correct me if im wrong.

[Dott. Berrino]

Perfect Chris4!!!

[2810712]

Oh I didn't know that formula, how many of us here really happen to use them? ? ?

I think 2 out of 5 , right ? ? ?

hrushikesh

[Chris4]

I used it. In my 1st and 2nd year of university. Everyone who was doing a biology related degree at the uni had to use it for the basic genetics and molecular biology practicals.

[2810712]

What ? I thought u are preuniv. I'm preuniversity student.
sorry this is not off topic forum,but Iwas just curious, sorry.

hrushikesh

[Chris4]

What ? I thought u are preuniv. I'm preuniversity student.
sorry this is not off topic forum,but Iwas just curious, sorry.

hrushikesh

Im new here. I've never mentioned anything about university before.
Im in my 3rd year of a 4 year Cell Biology degree. So ive only got just over a year left

[2810712]

best luck!!! cellbio is great ! ! !

hrushikesh