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seperate nucleotides by electrophoresis

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seperate nucleotides by electrophoresis

Postby goose_keeper » Fri Dec 30, 2005 12:53 pm

what is the best pH value when seperating AMP UMP CMP GMP in electrophoresis?

Thanks for your attention!
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use a slightly acidic pH

Postby Jumpshooter » Fri Dec 30, 2005 6:56 pm

to separate those nucleotides. Try using Tris EDTA buffer adjusted to pH = 6.25 as the mobile phase on your agarose gel.
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Postby jeffrey » Sat Dec 31, 2005 12:57 am

"Nucleic acids however, remain negative at any pH used for electrophoresis and in addition carry a fixed negative charge per unit length of molecule, provided by the PO4 group of each nucleotide of the the nucleic acid. Electrophoretic separation of nucleic acids therefore is strictly according to size."
so, my opinon is just give a PH which can not destroy these nucleotides
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Thanks!

Postby goose_keeper » Sat Dec 31, 2005 4:10 am

pH A, 1.5 B, 3.5
C, 6.5 D, 9.5
I don't know why and which one I should choose .
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