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COMPLIMENTATION TEST

Genetics as it applies to evolution, molecular biology, and medical aspects.

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COMPLIMENTATION TEST

Postby SHEKHAR » Tue Dec 27, 2005 7:29 pm

HOW CAN WE DETERMINE the cis-mutation & trans-mutation USING COMPLEMENTATION TEST?


thank u in advance for clues :)
shekhar
P.G.(BIOCHEM.)
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Postby Jessieh04 » Thu Dec 29, 2005 4:02 am

two mutants are crossed, and the F1 is analyzed. If th e F1 expresses the wild type phenotype, we conclude each mutation is in one of two possible genes necessary for the wild type phenotype. When it is shown that shown genetically that two (or more) genes control a phenotype, the genes are said to form a complementation group. Alternatively, if the F1 does not express the wild type phenotype, but rather a mutant phenotype, we conclude that both mutations occur in the same gene (cis)

Complementation tests allow us to see whether any given gene complements a mutant or fails to do so. If complementation occurs, the mutant must be in some other gene. If complementation fails to occur, the mutant is in the same gene.
Nothing lasts forever...so live it up, drink it down, laugh it off, take chances & never have regrets because at one point what you did was what you wanted.
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Postby SHEKHAR » Thu Dec 29, 2005 5:37 pm

THANKS JESSIEH.......... FOR UR COMMENTS .
IT WOULD HELP ME A LOT FOR MY STUDIES.
CAN U GIVE UR E-MAIL TO MY ID
shekhar_biochem@rediffmail.com
I WOULD WAIT FOR UR RSPONCE
THANKS AGAIN
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