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Questions about DNA dialysis

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Questions about DNA dialysis

Postby protoemics » Thu Feb 05, 2015 7:56 am

Dear all,
I would like to use dialysis to exchange ions in my DNA. For this I bought Slide-A-Lyzer mini pots for the dialysis.
First, I am looking for more information about the theoretical background of dialysis. Can you give me some literature hints, please?

Secondly, I know my target DNA concentration, but I know dialysis can lead to dilution and sample loss. How much higher shall I choose the starting concentration?
Also, how can I calculate the optimum time for dialysis, the right temperature, please?

Thank you very much for your hints.
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Postby leesajohnson » Tue Sep 13, 2016 8:28 am

Kidney function goes down and the body is not able to remove waste and excess water from the blood, dialysis is needed.
In dialysis the waste & excess water is removed from the body by using an artificial kidney. During kidney failure, dialysis is a life-support treatment that uses a special machine to filter harmful wastes, salt, and excess fluid from your blood. Dialysis replaces many of the kidney's important functions. It is typically a 3-4 hour process where blood is taken out from the body and passed through dialyser where the blood is filtered and then put back in the body. These processes can be done in a hospital or at home.
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