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What an expression vector must have

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What an expression vector must have

Postby olduvai » Sun May 03, 2015 6:48 am

Can You please help me with this question?
What MUST have a vector, that can exprime proteins in mammals cells?

Thanks in advance!:)
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Joined: Sun May 03, 2015 6:42 am

Postby Javedz2001 » Sun Oct 11, 2015 5:01 pm

The vector must have promoter (T7, lambda, lac etc. for E.coli), transcription start site and multiple cloning site for cloning your gene. Your target sequence must have ATG or should come from the vector. If you have to purify proteins then it should have some type of tag: his tag, etc.

Good Luck: Javed Siddiqi
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Joined: Sun Oct 11, 2015 4:32 pm

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