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Contamination in cells?

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Contamination in cells?

Postby LochNess » Tue Sep 08, 2015 7:33 pm

Hello. In the past, we had a contamination caused by E.coli in our lab. Now I am not sure, if our cells are ok or not. Could you please help me? I am attaching two photos, whta do you think about it?
Thanks for helping us.
Attachments
Gramovo obarveni.jpg
Gram coloration of our cells
Obarvene bunky crystal violet.jpg
Cells fixed and colored using crystal violet in methanol
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Postby Fornita » Wed Jun 01, 2016 3:34 am

I cannot watch clearly for the first picture, but the second one, the black dots look like pollution. Did them move when you watched them under microscope?
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Postby leesajohnson » Tue Sep 06, 2016 7:24 am

Contamination of cell cultures is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequences. Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera & water, end toxins, plasticizers , & detergents, & biological contaminants such as bacteria , Mold, yeasts, viruses, Mycoplasma as well as cross contamination by other cell lines. https://youtu.be/cyJ2zUbLMMo
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Postby biomind2017 » Mon Jan 23, 2017 1:27 pm

Agree with Fornita, bacterial contamination is easily detected by visual inspection of the culture within a few days of it becoming infected. Infected cultures usually appear cloudy (i.e., turbid), sometimes with a thin film on the surface. Under a low-power microscope, the bacteria appear as tiny, moving granules between the cells, and observation under a high-power microscope can resolve the shapes of individual bacteria.
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Postby joemasters » Thu Feb 02, 2017 3:41 pm

You will need a higher powered microscope, but I agree with the others, it appears as if there is some sort of bacterial contamination.
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