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Mutagenesis

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Mutagenesis

Postby Shaanu » Wed May 07, 2014 8:23 pm

Halo :) Can anyone explain in simple words what is en passant mutagenesis. How it
is different from usual methods of manipulating genomes.
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Re: Mutagenesis

Postby Shaanu » Sun May 11, 2014 11:01 am

???
No one knows .. OOps? :( :(
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Postby JackBean » Sun May 11, 2014 6:35 pm

http://www.ncbi.nlm.nih.gov/pubmed/20677001

First you use a sequence with the selection marker (as usually), but that is later excised by I-SceI cleavage and the second Red recombination.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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