Login

Join for Free!
118255 members


Restriction Digesion

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Restriction Digesion

Postby Helics » Sun Apr 06, 2014 4:07 pm

which is the best option for Restriction Endonuclease digesion ???

A) 37' C water bath
B) 37' C PCR program
Helics
Garter
Garter
 
Posts: 38
Joined: Fri Oct 12, 2012 9:10 am

Postby MOmarKhan » Mon Apr 07, 2014 2:09 am

Both of them are okay! But i would go for Thermo-cycler instead of water bath.
MOmarKhan
Garter
Garter
 
Posts: 1
Joined: Mon Apr 07, 2014 2:05 am

Postby Helics » Tue Apr 08, 2014 4:26 am

Dear scientist, Jack Bean I need your response.
Helics
Garter
Garter
 
Posts: 38
Joined: Fri Oct 12, 2012 9:10 am


Postby JackBean » Sun May 04, 2014 12:04 pm

thanks for considering me a scientist :-D

I don't see much difference if you're sure that the temperature iscorrectly set. However, with thermocycler (or thermoblock) you do not have to worry, that you will spill your samples :)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5678
Joined: Mon Sep 14, 2009 7:12 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests