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Gel Electrophoresis Help!

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Gel Electrophoresis Help!

Postby cks123 » Tue Aug 27, 2013 4:25 pm

So I did a double digest for subcloning and I didn't get two distinct bands when I ran the gel. Can someone interpret the gel pattern for me?
pI-SceI site digest.jpg
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Postby JackBean » Wed Aug 28, 2013 2:11 pm

not really, because there is hardly something visible. What sizes is it? What sizes did you expect?

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Postby bravebeaker » Thu Aug 29, 2013 7:14 am

size of your vector/insert.
gel is prestained with ethidium bromide or not.
what are the restriction enzymes you are using?
did you check if your vector contains multiple sites of the enzymes you are using?

The bands are way up, higher than 10k bp..
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Postby Cat » Sun Sep 01, 2013 2:23 pm

It looks like a bad gel. I suggest to re-run with fresh buffer/gel.
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