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molecular question.

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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molecular question.

Postby aapje10 » Mon Aug 19, 2013 5:43 pm

Hello people, I need this one for my homework but can't figure it out..

The question is:
Imagine you have a mutant but you don't know the region where the mutant gene is allocated. How would you find out where the gene is located by using a genomic bank?

I think it isn't possible with hybridization techniques because you dont have a primer? So im curious what the solution will be.

Thanks :)
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Postby JackBean » Tue Aug 20, 2013 1:50 pm

what organism? What mutation?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: molecular question.

Postby aapje10 » Tue Aug 20, 2013 8:21 pm

The question didn't mension what organism or what mutation, its just a question in general i guess. It comes from molecular principles (david hyde), since I already had my test, the question is irrelevant to me now anyway.
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Postby JackBean » Wed Aug 21, 2013 1:35 pm

And the answer?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby jwalin » Wed Aug 28, 2013 10:21 am

knowing the organism is crucial but given that its a general question a general answer would be that you would use a nucleotide blast (given that you have the gene sequence).
it isn't what you do that matters but it is how you do it
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Postby JackBean » Wed Aug 28, 2013 1:54 pm

I don't think that would be the case. I'd expect "some" mutation happening and then realizing where it happened.
http://www.biolib.cz/en/main/

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