Login

Join for Free!
117490 members


Another molecular question

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Another molecular question

Postby aapje10 » Mon Aug 19, 2013 6:03 pm

If you have a cell that is producing a lot of a given protein (such as hemoglobin in erythrocytes) and you want to isolate the gene that transcribes the mRNA for that protein, the method that would likely give you the best results would be

I just can't figure it out? Is there a way to go from protein to mRNA?
aapje10
Garter
Garter
 
Posts: 4
Joined: Mon Aug 19, 2013 5:34 pm

Postby JackBean » Tue Aug 20, 2013 1:52 pm

I'd say hybridization of cDNA library, but it would not work for your example ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5672
Joined: Mon Sep 14, 2009 7:12 pm

Re: Another molecular question

Postby aapje10 » Tue Aug 20, 2013 8:19 pm

If you have a protein, you can determine the amino acids. When you know these, you can figure out the nucleotide sequence. Next of you can hibridize a probe, and isolate your piece of dna maybe?
aapje10
Garter
Garter
 
Posts: 4
Joined: Mon Aug 19, 2013 5:34 pm


Postby JackBean » Wed Aug 21, 2013 1:37 pm

you will have degenerated sequence, because most of amino acids are coded by more than one triplet. But if you manage to select piece where are mostly AAs coded by only one or two codons, you could prepare degenerated probes and use them for the hybridization. However, that will not work for hemoglobin in erythrocytes.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5672
Joined: Mon Sep 14, 2009 7:12 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest

cron