Login

Join for Free!
118823 members


Culturing or viewing cells

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

Culturing or viewing cells

Postby NathanielZhu » Thu Aug 08, 2013 7:55 am

1. When scientists study blood under a microscope, do they usually add a chemical to keep the blood from clotting?
Every time I check out my blood from a nose bleed, it ends up clotting before I manage to get a nice look at it.

2. How do scientists culture cells like cancer? I've read that every cancer cell has a different set of ingredients, but is there a general procedure?

3. I'm studying to be a biochemistry, but I also want an overview of everything a medical doctor learns (just to be informed). Care to suggest a list of books/subjects studied by medical doctors?

4. How successful are multiple organ transplantation in humans?
Why Do We Age?
http://hubpages.com/hub/Theories-on-Aging
Nature vs Nurture in Animal Behavior
http://hubpages.com/hub/Biology-Innate-Behavior-in-Animals
Photosynthesis
http://hubpages.com/hub/Understanding-Light-Dependant-Photosynthesis
User avatar
NathanielZhu
Garter
Garter
 
Posts: 7
Joined: Fri Apr 29, 2011 4:22 am

Postby balamurugan3 » Sat Aug 10, 2013 3:17 am

I wish I could answer every questions but not...

1. collect blood always with EDTA, which chelates Calcium and prevents cloting.

other answers need some experts...
balamurugan3
Garter
Garter
 
Posts: 9
Joined: Wed Aug 07, 2013 2:29 pm

Postby jwalin » Fri Aug 30, 2013 2:22 pm

2. Different cancer cells will definitely require different media. But the general procedure assuming you need to get cells from a particular tissue would be to get the tissue, extract the cells, digest ECM..., try culturing them in a flask / Tissue culture plate (depending on the type of cells it can be determined if anchoring is required or not), make the cell specific media recipe and grow the cells.
The cells can then be subcultured for a limited number of times but they can be subcultured (cancer cells can be subcultured a lot more than the other well differentiated cells)
it isn't what you do that matters but it is how you do it
User avatar
jwalin
Coral
Coral
 
Posts: 438
Joined: Tue Sep 15, 2009 3:43 pm
Location: ahmedabad, gujarat, India


Postby agb06 » Fri Sep 13, 2013 2:21 am

Insuline is better than EDTA. You much quickly spin down the blood cell, otherwise it will have hemolysis.

___________________________________________________________________________________________
www.agenombio.com
agb06
Garter
Garter
 
Posts: 2
Joined: Fri Sep 13, 2013 2:01 am


Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 2 guests