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Getting Bacteria from an OD0.8 to an OD 0.1

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Getting Bacteria from an OD0.8 to an OD 0.1

Postby biology_06er » Sun Jun 16, 2013 12:15 pm

Hi there,

Say I have 50mL of bacteria at an OD0.8 and I need to get bacteria to a reading of 0.1..this is what I've been doing in the past but just want to confirm that what I've been doing in correct.

So from my 50mL, I took 1mL, spun and then resuspended the pellet in 1mL of PBS. (I now have 1mL of bacteria at OD0.8 in PBS)
I took 125uL from this and added to 875uL of PBS (OD has gone down 8x now)

Is this correct--would this now give me 1mL of bacteria at an OD0.1?

Thanks,
biology_06er

Exp: Incase you want to know why I need at OD0.1 it's because I want to add a certain about of bacteria to my assay..I know roughly how many are contained in an OD0.1 so from there I can add the correct volume ..
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Postby JackBean » Mon Jun 17, 2013 7:20 am

yes
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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