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Use of cDNA when studying splicing

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Use of cDNA when studying splicing

Postby nukeman » Wed May 15, 2013 11:33 am


I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples represented the non-spliced samples. cDNA is, as far as I know, made from mature mRNA which is fully spliced?

Could someone please explain this?

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Postby JackBean » Thu May 16, 2013 9:42 am

can you show some such studies?

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