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Use of cDNA when studying splicing

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Use of cDNA when studying splicing

Postby nukeman » Wed May 15, 2013 11:33 am

Hi

I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples represented the non-spliced samples. cDNA is, as far as I know, made from mature mRNA which is fully spliced?

Could someone please explain this?

Thanks
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Postby JackBean » Thu May 16, 2013 9:42 am

can you show some such studies?
http://www.biolib.cz/en/main/

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