Login

Join for Free!
116951 members


RNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

RNA

Postby 2468 » Tue Nov 20, 2012 6:03 pm

Ques1: Phosphorylation of RNA pol
which are the roles of the phosphorylaton of C terminal Domain of RNA polymerase? (more than one may apply)
1. It increases the speed of transcription
2. It increase the specificity of the transcription process
3. It pauses transcription to allow for capping to occur
4. It facilitates termination of transcription

3? not too sure which else...


Ques 2: Which may not be consequences of RNA splicing
1. Insertion of amino acids in the protein sequence
2. Deletion of amino acids in the protein sequence
3. Substitution of amino acids in the protein sequence
4. Formation of chimeric proteins

maybe 4?

Ques 3: What are the potential outcome of RNA editing in terms of protein sequence? (more than one may apply)

1. Single amino acid substitution
2. Single amino acid insertion
3. Single amino acid deletion
4. Early termination
5. none of the above

is 2,3 correct?



THNAK YOU!! :D
2468
Garter
Garter
 
Posts: 23
Joined: Thu Nov 01, 2012 12:48 pm

Postby JackBean » Tue Nov 20, 2012 8:41 pm

2) if you cut the mRNA, how can you gain amino acids?

3) how did you come to 2 and 3?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5667
Joined: Mon Sep 14, 2009 7:12 pm

Postby revision » Mon Nov 26, 2012 12:01 pm

I think the answer to question 2 is formation of chimeric proteins because insertion, deletion and substitution are possible consequences of RNA splicing.
revision
Garter
Garter
 
Posts: 10
Joined: Sun Nov 25, 2012 4:05 pm


Postby simina » Tue Mar 19, 2013 10:42 am

Hi! Can anyone explain me how RNA chain grow and what is the role of CTP and ATP in this process?? thanks!!
simina
Garter
Garter
 
Posts: 15
Joined: Wed Feb 13, 2013 4:15 pm

Postby JackBean » Tue Mar 19, 2013 4:35 pm

check out transcription. If you'll have more specific questions, feel free to come back and ask in a new thread.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5667
Joined: Mon Sep 14, 2009 7:12 pm

Postby simina » Wed Mar 20, 2013 11:13 am

I really do not understand the difference between the cis-acting sequences and trans-acting sequence. I read many definitions but is still not clear for me. Any idea of simple and easy explanation that I could understand?:D
simina
Garter
Garter
 
Posts: 15
Joined: Wed Feb 13, 2013 4:15 pm

Postby nukeman » Thu Mar 28, 2013 2:21 pm

Cis and trans-acting regulatory elements are in essence transcriptional regulators/promoter sequences that are categorized based on acting distance.

The lac operon in E.coli is a typical example of a cis-acting element. Promoter sequences that regulate expression of the genes contained within the operon are directly adjacent to the genes themselves.

Trans-acting elements on the other hand are in general not located in the immediate vicinity of the target genes, but can instead be proteins encoded on completely different regions and thus target genes and regulatory sequences (e.g. certain cis-elements) that are far away, hence the terms 'Trans' (=across) and 'Cis' (=on the same side) (sort of..).

Recommended read: http://www.ndsu.edu/pubweb/~mcclean/pls ... trans6.htm
nukeman
Garter
Garter
 
Posts: 19
Joined: Wed May 23, 2012 4:11 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests

cron