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dot blot

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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dot blot

Postby Bio2013 » Thu Mar 14, 2013 6:07 am

Does any body have any experience with ''dot blot''?
http://www.bio-rad.com/prd/en/US/LSR/PD ... -Apparatus
thanks
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Postby JackBean » Thu Mar 14, 2013 2:25 pm

Little bit, yes.
http://www.biolib.cz/en/main/

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Postby Bio2013 » Sat Mar 16, 2013 5:12 pm

I am having problems with that. I am using Nitrocellulose membrane, at the beginning I was getting bleeding and leakage between the wells, next I saw shadow around the dots and now I am getting no signal.
any similar experience?
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Postby JackBean » Mon Mar 18, 2013 3:21 pm

I had problems with bubbles, so I didn't use it much, but I think someone else made it running...
http://www.biolib.cz/en/main/

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