Login

Join for Free!
119271 members


SDS-PAGE gel and Rf values

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

SDS-PAGE gel and Rf values

Postby cschauss » Sun Mar 03, 2013 7:37 am

Hello,
I have to calculate the Rf for the molecular weight markers in lane one of the gel that I attached. When I do this and graph the Rf values against the molecular weight my graph is not linear. I marked in red where I am measuring from. Can someone please tell me what I am doing wrong?
Attachments
cellbiogelll.png
cschauss
Garter
Garter
 
Posts: 2
Joined: Sun Mar 03, 2013 7:32 am

Re: SDS-PAGE gel and Rf values

Postby cschauss » Sun Mar 03, 2013 7:39 am

Also, here are my Rf values (in the x column):
0.0068 250000
0.021 150000
0.048 100000
0.075 75000
0.15 50000
0.24 37000
0.4 25000
0.49 20000
0.67 15000
0.9 10000
cschauss
Garter
Garter
 
Posts: 2
Joined: Sun Mar 03, 2013 7:32 am

Postby JackBean » Sun Mar 03, 2013 9:35 am

You should measure from the very top of the gel. Did you plot it against log of MW?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm



Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 1 guest