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How does a plasmid work?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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How does a plasmid work?

Postby WizardofScience » Sun Dec 30, 2012 3:05 am

I'm having difficulty understanding how exactly a plasmid does what it does. I know that it's generally located in bacteria, and it's non-essential to them. I don't understand how they replicate though. Does it work the same way as a bacterium's chromosomal DNA?

Thanks for any help!
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Postby JackBean » Sun Dec 30, 2012 10:25 am

Basically yes, but the replication start is not that much strict (thus you can have up to several hundreds of copies in one cell).
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby WizardofScience » Sun Dec 30, 2012 2:06 pm

Can you (or anybody else) go into a little more detail about how the replication works? Or link me to a website? Is it still the same with helicase unwinding the DNA and primase coming in and etc. like in regular DNA replication?
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