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Postby 2468 » Wed Nov 28, 2012 12:40 am

Consider the reference:

Chen, H., Hopper, S.L. and Cerniglia, C.E. (2005) Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein. Microbiology,151, 1433-1441.

This is:

a. A good reference for both the pKa and molar extinction coefficient of methyl red at 500nm

b. A reference for the molar extinction coefficient of the acid form of methyl red but it isn't exactly at 500nm

c. A good reference for the molar extinction coefficient of the acid form of methyl red at 500nm

d. A good reference for the pKa of methyl red

e. A reference that quotes an extinction coefficient for the base form of methyl red at 430nm and so not directly relevant to this study


http://mic.sgmjournals.org/content/151/5/1433.long


I think it might be 'C'?
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Postby JackBean » Wed Nov 28, 2012 9:45 pm

I found in the article only one mention of extinction coefficient (of FMN) and no pK
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby 2468 » Wed Nov 28, 2012 10:06 pm

yes, thats what i found too...so not too sure which answer its suppose to be exactly :/
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