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How do I create the sequence contig of this DNA?Moderator: BioTeam
3 posts • Page 1 of 1
How do I create the sequence contig of this DNA?You have the following sequence reads from the protein-encoding region of a Homo sapiens cDNA clone:
Read 1: tattatcctgactttagccatgaatatcatgagtacattgcagtgggctgttaactccagcataga Read 2: caaccaaaccatacaagaatggccaactctcgaaagttatgattattgagaattcacacgtg Read 3: agttatgattattgagaattcacacgtgaagaaagatgacatctggccctcagggggtcaaa Read 4: ggctgttaactccagcatagatgtggatagcttgatgcgatctgtgagccgagtctttaagttcattgaca Read 5: gaagaaagatgacatctggccctcagggggtcaaatgactgtcaaagatctcaca Read 6: tctttaagttcattgacatgccaacagaaggtaaacctaccaagtcaaccaaaccatacaagaatggc Read 7: atttccattttaacaacaggagaaggagaaggaagagttggtattatcctgactttagccatgaa c. Use these 7 sequence reads to create a sequence contig of this portion of the H. sapiens cDNA. Show clearly how you achieved this. I don't understand what's going on, help me to understand what this DNA sequence is supposed to tell me and how I sequence contig? After this I have to convert it to possible reading frames.
Re: How do I create the sequence contig of this DNA?Look for overlapping sequences. For example, Read 1 begins with tattat. Does this sequence occur in any of the other reads?
3 posts • Page 1 of 1
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