24 wells plate for migration assay

[Halaqataruniversity]

Hey Guys,
I would like to know how much VSMC cells you need in the 24 multiwells insert system, or they call it now the modified boyden chamber. for migration assay

P.S:
I searched online and found that some protocols had used between 30,000 cells - 50, 000 cells. well, I got confued because I use 40,000 cells in 6 wells plate!!! and the 24 wells plate is smaller in size? so howcome?!?!

If anyone had tried it before let me know

Regards