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low levels of protein but high activity levels

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low levels of protein but high activity levels

Postby sns » Thu Nov 08, 2012 4:08 pm

Hi,
I am stumped on one of my projects. In my experiment I found that the gene expression is high, protein levels low but activity levels are high for this enzyme in diseased samples compared to age-matched controls. Has anyone else seen anything like this?
sns
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Postby JackBean » Thu Nov 08, 2012 7:15 pm

No, nobody.

Now seriously. Do you know what is the specific activity of your pure enzyme? That could tell you something. How do you determine the protein level?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby sns » Mon Nov 12, 2012 8:00 pm

I determined the protein levels using westerns
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Postby JackBean » Mon Nov 12, 2012 11:29 pm

the antibody was against tag or your protein?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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