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Dilution problem for my Bradfords assay

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Dilution problem for my Bradfords assay

Postby moabubaker » Fri Oct 19, 2012 11:55 am

Can someone help me understand why a sample has a final concentration of 1500ug/ml. If I add 2mg/ml of stock, 10ul of water and 150ul of bradfords reagent.

If someone can point me in the right direction of how they worked it out. Thanks a lot.
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Postby JackBean » Fri Oct 19, 2012 12:09 pm

you need some calibration curve to calculate the amount of proteins in your sample
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Dilution problem for my Bradfords assay

Postby moabubaker » Fri Oct 19, 2012 12:33 pm

No, this is one of the standards I used to construct the absorbance/conc curve. I'm just wondering if you can help me figure out how adding 2mg/ml BSA stock with 10ul diluent and 150ul of bradford reagent gives a final BSA conc of 1500ug/ml. I suck at maths and im having a hard time understanding how this was calculated.
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Postby JackBean » Fri Oct 19, 2012 1:01 pm

cV = cV
unless you tell me how much of your sample did you add, I will hardly help you
http://www.biolib.cz/en/main/

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