Biology-Online • View topic - Gram Positive Cocci

Join for Free!
122619 members

Gram Positive Cocci

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Gram Positive Cocci

Postby lucrezia03 » Wed Oct 17, 2012 11:58 pm

I am working on a biology research project to identify a bacterial organism found in a small stream. Here is the info collected so far:

Colony morphology: Yellow, round, slightly concave, smooth.

Gram stain: Gram positive, appears to be cocci, but not perfectly round and tend to group together in pairs (which appear as though it is a short fat bacilli) or irregular clusters.

Blood Agar: Gamma hemolytic

EMB: Colorless growth (does not ferment lactose)

MS Agar: No growth

TSI: Yellow butt, yellow slant (ferments glucose, lactose and/or sucrose) (I am also wondering does this mean it does ferment lactose? Or can it mean that it ferments sucrose instead of lactose?)

Nitrate Reduction: Negative (does not break down NO)

Catalase: Positive

Based on the first dichotomous key we had been using it led us to a bacteria called Gaffkya Tetragena, which I could not find any information on except for some very old medical journals (from the 30's and 50's). I found one page that mentioned something eluding to the fact that this species was renamed/reclassified to Aerococcus Viridans, which does not sound like it matches up with our organism. I looked at another bacterial flow chart and it led to micrococcus verians, and when I looked that up there also was not a lot of info, but mostly I found references to the fact that the micrococcus genus had been dissected into a new genus called Kocuria. I have also looked up this name, and cannot seem to find much information. If anyone has any advice on how I can find more info about this organism, tests we could try, or any other suggestions they would be much appreciated.
Posts: 1
Joined: Wed Oct 17, 2012 11:36 pm

Postby Cat » Fri Oct 19, 2012 12:47 am

16S rDNA sequencing.
King Cobra
King Cobra
Posts: 635
Joined: Thu Feb 14, 2008 7:40 pm

Postby ohyesitskim » Sun Oct 28, 2012 6:34 am

Hey your bacteria might be micrococcus luteus or something which is the same as micrococcus Kocuria, I believe. Try using the Bergy's manual of determinative bacteriology. Good luck and if you can take a look at mine and help me out that would be great!
Posts: 3
Joined: Mon Oct 15, 2012 10:21 pm

Return to Microbiology

Who is online

Users browsing this forum: No registered users and 0 guests