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RFLPs

Genetics as it applies to evolution, molecular biology, and medical aspects.

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RFLPs

Postby dreamtim » Wed Oct 03, 2012 2:05 pm

Hallo

I'm having hard time understand an RFLP anlaysis.
I've attached a beautiful sketch I did.

1.What represent the letters A/B under each descendant?
2.The restriction enzyme is added the F2? how come there is just one blue and red band? if it cut it to two pieces..
3.Why in M2 there two red bands?

I've been trying to understand it for hours!!
Thank you!
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Re: RFLPs

Postby dreamtim » Tue Oct 09, 2012 10:09 am

Is the sketch isn't clear? or the questions?
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Postby JackBean » Tue Oct 09, 2012 10:13 am

That seems like lots of mess. For example, why should in F1 be anybody with BB (two blues)? Gametes do not have two pairs of chromosomes. What is M1, M2?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: RFLPs

Postby dreamtim » Wed Oct 10, 2012 8:56 pm

F1 progeny is in the left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme.
The bottom table shows the gel-electropohresis analyse. The table gametes shows the 4 possible gametes between F1 progeny(red and blue) and blue-blue.
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