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RFLPsModerator: BioTeam
4 posts • Page 1 of 1
RFLPsHallo
I'm having hard time understand an RFLP anlaysis. I've attached a beautiful sketch I did. 1.What represent the letters A/B under each descendant? 2.The restriction enzyme is added the F2? how come there is just one blue and red band? if it cut it to two pieces.. 3.Why in M2 there two red bands? I've been trying to understand it for hours!! Thank you!
Re: RFLPsF1 progeny is in the left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme.
The bottom table shows the gel-electropohresis analyse. The table gametes shows the 4 possible gametes between F1 progeny(red and blue) and blue-blue.
4 posts • Page 1 of 1
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