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Maing wet mounts

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Maing wet mounts

Postby Bioman » Tue Feb 15, 2005 8:06 am

How to prepare a wet mound slide. The specimen is an onion skin. :D
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Postby mith » Tue Feb 15, 2005 8:26 pm

Basically make it so the specimen is in the slide and fill up the excess space with water. Then cover with coverslip. I don't know why you would do this though if it's onion, you can view it normally I think.
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Postby biostudent84 » Tue Feb 15, 2005 8:34 pm

Place a drop of water on the slide. Carefully place the onion skin in this drop of water. Make sure it does not fold over. If it does, use a probe to straighten it out. Take the coverslip and place the edge of it in the water to one side of the onion skin. Slowly lower the other side of the coverslip to the slide. This will reduce tha appearance of air bubbles in the water.

***Air bubbles appear as white circles outlined by a thick black line***

This is a common practise in biology in American schools, Mithrilhack, it teaches students how to properly use a microscope for future work.
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