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pcr purification

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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pcr purification

Postby siddharthsameer » Wed Aug 01, 2012 5:41 pm

Hello
the concentration of my PCR product before purification was 1000microgram/ml and after the purification of my PCR product it has come down to 33.28 microgram/ ml.. what does it indicate.. is everyhing going fine or there is some problem in my pcr product..
kindly tell me....I am a big confusion. kindly help me..
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Postby JackBean » Wed Aug 01, 2012 6:25 pm

how did you measure the concentration? By UV at 260 in PCR reaction? There are all the nucleotides and oligos, which absorb just as much, thus so high concentration ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby siddharthsameer » Wed Aug 01, 2012 6:54 pm

heelo i measured through UV spectro, but the concentration is too low,,,,do u know wats the reason
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