Biology-Online • View topic - Restriction Digestion important
Login

Join for Free!
121770 members
Advertisement
Advertisement
Advertisement


Restriction Digestion important

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Restriction Digestion important

Postby siddharthsameer » Wed Aug 01, 2012 4:17 pm

hello I need to ask one question and I am stuck with this kind;y help me...
The concentration of my pcr product is 33.56microgram/ml and I want to make the restriction digestion .I want to have the 2 microgram of my product in the ristriction digestion volume of 50microlitre. How much volume should i add of my pcr product to the reaction volume for the restriction...
kindly let me know I am stuckk and can not find the answer....please please help me
siddharthsameer
Garter
Garter
 
Posts: 22
Joined: Sun Jul 15, 2012 6:36 am

Postby JackBean » Thu Aug 02, 2012 7:26 am

let's say it's 30 ng/ul and you want 2000 ng, how many-times is it? 2000/30 ~60-times -> thus you would need 60 ul
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest

Advertisement