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How to calculate mass of target Dna in ng

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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How to calculate mass of target Dna in ng

Postby siddharthsameer » Wed Jul 18, 2012 7:37 am

hello Everyone
I have a small question, how do I calculate the mass of target DNA in ng as I am looking to calculate the amount of Restriction enzyme required for the digestion of my target DNA.Or How should I calculate the Volume needed for Restriction enzyme to digest my Vector And Target Dna.
I have only the concentartion of my Target DNA AND Vector and as well as the base pair. are these information sufficient to calculate the volume or do I need tio have more information for the calculation.
Kindly help me I am stuck with this problem..
with regards
siddharthsameer
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Postby genetherapy » Mon Jul 30, 2012 11:25 am

Look at this web-sites,the informations that they have will help you

http://www.molecularstation.com/dna/res ... digestion/
http://www.methodbook.net/dna/restrdig.html
genetherapy
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