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HL60 cells centrifugation

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HL60 cells centrifugation

Postby claire84 » Wed Jul 04, 2012 3:58 pm

Hi everyone!
I need your help because I have little experience on cell culture techniques...I would centrifuge my Hl60 cells which are plated in a 96-well microplate, to obtain a pellet at the bottom and to remove supernatant without loss of cells.
Could you recommend me rpm and time to use?
I tryed with 1500 rpm for 5 minutes, but I think I need to increase the time...
thank so much

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Postby JackBean » Thu Jul 19, 2012 6:44 am

5000 g for 1 minute is used for bacteria and I don't think it would be much different for other cells.

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