Flow cytometry-antibody conc.

[biology_06er]

Hi there,

I'll be doing FC for the first time next week, and I have a protocol but I'm slightly confused about one thing...after I resuspend my cells in PBS/BSA it tells me to take 200uL of cells and incubate with 1:200 of primary antibody...so does this mean if I have 200uL of cells, I add 1uL of my antibody...and then if I wanted to to another conc of antibody say 1:400 I would add 0.5uL of antibody...I recall being told I would have to do serial dilutions of my primary antibody..but how do I go about doing serial dilutions..if i need 200uL of cells to start with?

Hope I make sense!
cheers,
b_06er

[biology_06er]

Problem solved!