What stats test should I use?

[biology_06er]

Hi there,



I recently did an ELISA and have no idea what type of stats test to use?...I haven't done a single stats paper/stats related anything in over 6years..so am struggling to analyze my current results.



I basically did the standard elisa,,,,adding various amounts of protein to coated wells...and i did triplicate wells for each concentration...so i guess what i would like to know if there is a a stat. sig difference between the highly concentrated and less concentrated samples...



anyhelp will be greatly appreciated..preferably in a language i can understand!



cheers,

b_06er

[biology_06er]

anyone? I tried doing the RM anova but it came out as sig..but clearly I had no binding..soo what am I doing wrong..essentially the -ve control values were the same as by test readings..the curve shows a very very slighly increase from lowest conc. to highest conc. If i wanted to test if there was a diff. between highest/lowest comp..seems an Anova isn't working