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Plant hormone experiment!

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Plant hormone experiment!

Postby duherro » Mon May 07, 2012 7:07 am

Got given a practice prac on plant hormones.

Method:
1. Place 8 corn seeds (with 2 each facing towards the 4 poles, so North, East, South, West).

2. Cover seeds with a piece of filter paper

3. Pack petri dish with moist cotton wool.

4. Put lid on the petri dish and seal it with sticky tape.

5. Stand petri dish upright attached on plasticine and near a window.

6. Let it stand for 1 week and observe.



Q1 )The question asked if the above was a controlled experiment or not.
I think that it was not a controlled experiment as there weren't any variables subject to any change. (Not too sure about this question). However, maybe the north and south placed seeds could be the controls to show the direction of growth of shoots?


Q2) What differences in growth were visible over the period of the investigation? And suggest any reasons for any differences of growth and explain how it could be altered.
The shoots all grew vertical, while the roots will grow down.
auxin ?



Need help on writing an aim and hypothesis!
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Postby JackBean » Mon May 07, 2012 7:58 am

so did the seeds stay oriented to the two poles (there are no poles in East and West :roll:)? Or did you change it?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby duherro » Mon May 07, 2012 9:24 am

JackBean wrote:so did the seeds stay oriented to the two poles (there are no poles in East and West :roll:)? Or did you change it?


Like on the petri dish , imagine there are 4 points, N E S W. And in each of those directions we put 2 seeds in there. Btw, do you hhave any IM ?
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Postby JackBean » Mon May 07, 2012 10:09 am

yeah, I understand that. Does the Petri dish stay as it is or is it turned to be vertical or upside-down? What's the aim of the experiment?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby duherro » Mon May 07, 2012 10:46 am

JackBean wrote:yeah, I understand that. Does the Petri dish stay as it is or is it turned to be vertical or upside-down? What's the aim of the experiment?



The petri dish is just upright and left alone for 1 week. The aim is most likely to observe the growth reponse from seeds to light and gravity ? Got skype or something?
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Postby JackBean » Mon May 07, 2012 11:01 am

And how does gravity differ depending on whether is the seed facing toward North or West?

Sorry, I don't have Skype or anything
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby duherro » Mon May 07, 2012 11:18 am

JackBean wrote:And how does gravity differ depending on whether is the seed facing toward North or West?

Sorry, I don't have Skype or anything



Well just imagine it will be like

>N
W E
>S

and in each of the poles, there are 2 seeds in them
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Postby JackBean » Mon May 07, 2012 11:40 am

Is it vertical or horizontal?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby duherro » Mon May 07, 2012 12:05 pm

JackBean wrote:Is it vertical or horizontal?


Its facing upright, so vertical


like standing up , your head is the N, your arms are E and W and your feet are S. sorta like that
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Postby JackBean » Mon May 07, 2012 12:09 pm

OK, so the West, East, North and South are only for confusion...

Yep, in such case you're researching the effect of gravity. And yes, auxin flux is responsible for that.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re:

Postby duherro » Mon May 07, 2012 12:23 pm

JackBean wrote:OK, so the West, East, North and South are only for confusion...

Yep, in such case you're researching the effect of gravity. And yes, auxin flux is responsible for that.



could you suggest me a possible hypothesis?

And also...

Suggest a biomolecular reason for any diffrences observed in the growth of plant and explain how the growth was alterted.
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Postby JackBean » Mon May 07, 2012 12:57 pm

Hypothesis why or how?

The biomolecular reason here was the auxin and PIN proteins :)
http://www.biolib.cz/en/main/

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