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indole

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indole

Postby michimichi » Tue Apr 24, 2012 1:33 am

can i confidently detect an e.coli in water just by performing the indole test alone?
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Postby JorgeLobo » Tue Apr 24, 2012 2:37 am

No - you'd have to develop a test for indole in mixed culture of direct water inoculation and other bacteria produce indole (from tryptophane) including Vibrio, Pleisomonas, Salmonella, Citrobacter spp.
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Postby michimichi » Tue Apr 24, 2012 3:49 am

The indole test was done ofcourse after the presumptive to complete the test, so what i should do is perform further biochemical test other than indole to identify an e.coli. Is API 20E more realible than IMVIC test?
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Re: indole

Postby JorgeLobo » Tue Apr 24, 2012 8:38 am

Presumptive? Sorry, I thought you were suggesting unique protocol to identify directly presence of E. coli in water. What is the purpose then? If a one-time experiment, use API.
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Postby michimichi » Tue Apr 24, 2012 2:58 pm

ah ok, its not practical to use API always,,, its more expensive than the IMVIC but API can give rapid results..thnks sir
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Re: indole

Postby JorgeLobo » Tue Apr 24, 2012 3:07 pm

You could do it the old way - smell, colony morphology on EMB and Mac
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