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How to measure relative mobility of DNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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How to measure relative mobility of DNA

Postby Bioc » Fri Apr 06, 2012 2:56 am

Hi, I need to calculate the weight of different fragments of DNA in gel electrophoresis, using a calibration curve. My question is: What do I take as the reference point for the distance traveled by the band? the top, middle or bottom? (red, green, blue line)
Please help.
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Postby underwzc » Sat Apr 07, 2012 5:22 am

You would use the blue length because it allows for consistant measurement of all lanes in the gel.
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Re: How to measure relative mobility of DNA

Postby Bioc » Sat Apr 07, 2012 7:18 pm

Thank you.
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Postby JackBean » Mon Apr 09, 2012 4:17 pm

just use the same for all bands :)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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