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Inhibitor

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Inhibitor

Postby WiNtErSuMmErAuTuM » Mon Mar 19, 2012 12:29 am

Does anyone know how to tell if a inhibitor is competitive or non competitive ? specifically thyroxine ??
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Postby canalon » Mon Mar 19, 2012 1:35 am

Experimentally try multiple reactions with a fixed amount of enzymes and inhibitor and increasing concentration of enzymes and measure reaction rate. The results will tell you whether your inhibition is competitive or not (I let you figure out how).
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby JackBean » Mon Mar 19, 2012 1:25 pm

canalon meant constant concentration of enzyme and inhibitor and different concentrations of substrate ;)
Anyway, you can change concentration of both inhibitor and substrate.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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