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Inducing bacterial expression at a higher OD

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Inducing bacterial expression at a higher OD

Postby biology_06er » Thu Feb 23, 2012 9:54 am

Hi there,

I usually induce my for protein expression when the OD of my bacteria is 0-4-0.5. Seeing as there was no expression, someone suggested I inuduce at a higher OD...such as 0.8-1..why is this...because theres more bacteria at that stage??...

Thanks
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Postby canalon » Fri Feb 24, 2012 2:42 am

Mostly, yes.
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