Antibody Reconstitution

[knclayton]

Hi All,

I have question regarding some antibodies I wish to reconstitute.

The instruction form that came with the order states, "Reconstitute with 0.1 ml sterile distilled water (Final concentration=1mg/ml. Final buffer = 0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)"

So, does this mean I just use sterile distilled water, or am I somehow supposed to use PBS/BSA/etc., or is the PBS/BSA/etc. just part of the current makeup of the antibody vial that I have purchased?

Thanks!

[canalon]

Sounds to me like thr PBS/BSA/... are in the vial, and that by adding the H2O you will have the solution described.