Join for Free!
121591 members

Protoplast Isolation - first step - enzyme digestion

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Protoplast Isolation - first step - enzyme digestion

Postby bravebeaker » Fri Dec 16, 2011 9:02 am

Hello all,
I have been reading lots of papers about protoplast isolation. I found that most of the papers but not all say that enzyme digestion is in the dark. Why should it be in the dark? i have been trying to get an explanation but cant find any. In the mean time I am trying to enzyme digestion with and without light and see the difference but whats the purpose of digestion in dark conditions from 2 to 3 hrs?

AHH, Gravity! Such a cold cruel mistress.
User avatar
Posts: 44
Joined: Sun May 08, 2011 3:26 am
Location: Kobe, Japan

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 3 guests