RxFISH painting of chromosomes

[mzoltan22]

does RxFISH painting, disrupts the dna? so that it would cause damage to the genetic material? I read that it is some sort of combination of homologoues regions from non human,forreign dna made fluorescent. Are those simple weak hydrogen bonds mostly? if so, i bet it is easy to clear of the forreign dna. but, is it all doable in the living cell? so that chromosomes can sit there?

also Is it easy to put back condensed chromosomes into human cell nucleuses? (i bet it should be possible, but they have quite a considerable size).

[JackBean]

do you think uncondensed chromosomes are smaller?