electroporation

[JackBean]

oh man, you're part of EU, right? My apologies

[canalon]

I have to say that it is hard to see much from the picture, but if you have managed to generate nice square shocks with the possibility to control the intensity and duration, kudos to you.

[ZEGSUS]

Yes , I have.It was difficult but after many attempts I succeeded.

[ZEGSUS]

And what about plants?How to insert DNA from plant to plant without plasmids?

[JackBean]

for that you would need golden (or from other heavy metal) microparticles with use high pressure to bombard the plant tissue.
http://www.nepadbiosafety.net/for-regul ... ombardment

[ZEGSUS]

I know that but is there a chance to insert a genomic DNA with only electricty?

[JackBean]

I highly doubt so, because the plant cells have the cell wall, which is pretty good barrier for such transformation. Better way is to use Agrobacterium, which infects plants.
You want to insert full gDNA again?

[ZEGSUS]

Yes.Ok I will use Agrobacterium.Is it possible to transfer gDNA form plant to Agrobacterium?

[JackBean]

I though that since you have banned gene manipulation in Bulgaria, you won't have access to Agrobacterium neither. That's why I didn't mention it.
However, you cannot insert full gDNA using Agrobacterium, since plant genomes consist of several chromosomes and the Agrobacterium transfers only "small" portion from its Ti-plasmid. Nowadays there are available kits for Agrobacterium, which work with two plasmids, thus the inserted DNA may be longer, but it still won't be full chromosome.
(and I really don't see any reason, why to do that)

[ZEGSUS]

Well.Lets just say that I have Agrobacterium from somewhere.And thanks about information.

[JackBean]

you're welcome