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electroporation

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Postby JackBean » Mon Nov 07, 2011 8:48 am

oh man, you're part of EU, right? :roll: :oops: My apologies :(
http://www.biolib.cz/en/main/

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Postby canalon » Tue Nov 08, 2011 1:56 am

I have to say that it is hard to see much from the picture, but if you have managed to generate nice square shocks with the possibility to control the intensity and duration, kudos to you.
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any proof. (Ashley Montague)
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Postby ZEGSUS » Tue Nov 08, 2011 5:11 am

Yes , I have.It was difficult but after many attempts I succeeded.
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Postby ZEGSUS » Tue Nov 08, 2011 7:31 pm

And what about plants?How to insert DNA from plant to plant without plasmids?
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Postby JackBean » Tue Nov 08, 2011 7:45 pm

for that you would need golden (or from other heavy metal) microparticles with use high pressure to bombard the plant tissue.
http://www.nepadbiosafety.net/for-regul ... ombardment
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Postby ZEGSUS » Tue Nov 08, 2011 8:04 pm

I know that but is there a chance to insert a genomic DNA with only electricty?
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Postby JackBean » Wed Nov 09, 2011 5:39 pm

I highly doubt so, because the plant cells have the cell wall, which is pretty good barrier for such transformation. Better way is to use Agrobacterium, which infects plants.
You want to insert full gDNA again?
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Postby ZEGSUS » Wed Nov 09, 2011 6:08 pm

Yes.Ok I will use Agrobacterium.Is it possible to transfer gDNA form plant to Agrobacterium?
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Postby JackBean » Thu Nov 10, 2011 7:50 am

I though that since you have banned gene manipulation in Bulgaria, you won't have access to Agrobacterium neither. That's why I didn't mention it.
However, you cannot insert full gDNA using Agrobacterium, since plant genomes consist of several chromosomes and the Agrobacterium transfers only "small" portion from its Ti-plasmid. Nowadays there are available kits for Agrobacterium, which work with two plasmids, thus the inserted DNA may be longer, but it still won't be full chromosome.
(and I really don't see any reason, why to do that)
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Postby ZEGSUS » Thu Nov 10, 2011 2:42 pm

Well.Lets just say that I have Agrobacterium from somewhere.And thanks about information.
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Postby JackBean » Thu Nov 10, 2011 4:00 pm

:lol: you're welcome :)
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