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Which stain is frequently used to identify Mycobacterium?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Which stain is frequently used to identify Mycobacterium?

Postby SWEngineer » Fri Oct 28, 2011 10:27 am

Which stain is frequently used to identify Mycobacterium? Why is that? Is it to do with the high amounts of lipids contained in the cell wall?

Thanks.
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Postby aptitude » Fri Oct 28, 2011 6:51 pm

Mycobacterium is acid-fast because of the high concentration of mycolic acids (fatty acids) in the cell wall. They will appear to be red.
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Postby JorgeLobo » Sun Oct 30, 2011 2:33 am

Ziehl Neelson and its modifications.
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Re:

Postby SWEngineer » Sun Oct 30, 2011 11:40 am

JorgeLobo wrote:Ziehl Neelson and its modifications.


Yes, you mean Ziehl–Neelsen stain.

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