Genetics as it applies to evolution, molecular biology, and medical aspects.
here http://en.wikipedia.org/wiki/Bacterial_ ... chromosome they put the common size of insert into BAC is up to 350 kbp. Thus the number of clones you had to test would be N = [ln(1-P)]/[ln(1-[350/2300])] where P is the probability you will find your gene of interest or whethever are you looking for (99.5%) and 350/2300 is the ratio of insert and whole genome size. As I calculated it the N is ~4, what is odd, since the ration genome/insert is 6.5
I guess best to contact someone who is experienced e.g. someone from some sequencing company/group.
What is your aim again?
Cis or trans? That's what matters.
So you mean to cut the gDNA and to attach it to F-plasmids and after that to insert the plasmids into E.coli?
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