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What type of dna is needed in order to clone an animal?

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What type of dna is needed in order to clone an animal?

Postby Dab1493 » Mon Aug 29, 2011 9:06 am

Hello everyone, this is the first time i use a forum, and i don't know quite well how it works.
The reason i came here is because i have a question related to biology. I was wondering where exactly do they get the dna from, in order to successfully clone a human or animal. Also is it a quick process to exctract and store the dna from the donor?
Please explain the process.
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Postby JackBean » Mon Aug 29, 2011 9:12 am

I think that usually is transfered whole nucleus from some somatic cell into new denucleated egg cell
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby Dab1493 » Mon Aug 29, 2011 6:32 pm

that was a great reply. thanks
but can anybody explain a little more the process that takes place, and all the equipment needed.
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Postby Dab1493 » Thu Oct 20, 2011 2:38 pm

Please anybody caring to answer, i have many questions about cloning, maybe somebody could throw a quick chat with me.
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Postby Cat » Thu Oct 20, 2011 6:14 pm

Please, read the following:

http://www.synapses.co.uk/science/clone.html

While I am no expert on animal cloning, I will be happy to chat...
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Re: What type of dna is needed in order to clone an animal?

Postby Dab1493 » Sat Oct 22, 2011 6:57 pm

Thanks, i will take a look at it. :D
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Postby classificationking » Sun Oct 23, 2011 8:59 am

so by enucleating a cell and inserting a somatic cell's DNA this can be cloned
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Postby Dab1493 » Sun Oct 23, 2011 3:14 pm

Thanks classificationking for simplifying it, but why did this worked with Dolly and currently works with other animals, but doesn't work for humans?
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Postby JackBean » Sun Oct 23, 2011 3:46 pm

it could work, but there are ethical and legal issues ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby Cat » Sun Oct 23, 2011 7:38 pm

Not only that. There is an unexplained difference in the difficulty of "stripping" differentiation characteristics from the somatic nuclei of different species. For example, it's easier to work with cats than dogs...
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Postby JackBean » Sun Oct 23, 2011 7:46 pm

yeah, but until we try, we won't know. It may be easier to work with humans than sheeps. But we cannot find out.
http://www.biolib.cz/en/main/

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Postby Cat » Mon Oct 24, 2011 1:06 am

true
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