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ANNTI FUNGI !

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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ANNTI FUNGI !

Postby Nmr » Sat Sep 17, 2011 2:14 pm

HI GUYS...

WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?

COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !

THANK YOU
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Postby JackBean » Sat Sep 17, 2011 2:27 pm

1) don't shout on us
2) I think that pH should be quite enough. Further you can use some antibiotic.
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Postby canalon » Mon Sep 19, 2011 7:18 pm

Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.
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Postby choozi » Sat Oct 22, 2011 3:26 pm

YOU CAN USE NYSTATIN OR AMPHOTERICIN-B AS ANTIFUNGAL.
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Re: ANNTI FUNGI !

Postby JorgeLobo » Sat Oct 22, 2011 6:18 pm

Agree with canalon. If you can;t maintain aseptic technique and pure cultures - get out of the micro lab.

I'll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.
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Re: ANNTI FUNGI !

Postby daniel.kurz » Mon Oct 24, 2011 12:46 am

JorgeLobo wrote:Agree with canalon. If you can;t maintain aseptic technique and pure cultures - get out of the micro lab.

I'll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.


I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible...
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