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unknown bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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unknown bacteria

Postby 5646matthews » Thu Jul 16, 2009 10:38 pm

Hello,
I need help with an unknown bacteria and I am running out of resources. If anyone has the expertise or the time I would greatly appreciate any input. I have a gram neg rod shaped bacteria that is motile and facultatively anaerobic. It performs well at 37 C I have + results for acid and gas with Glucose, Sucrose, Lactose, and maltose. I have a + for citrate utilization and catalase. Negative on gelatin hydrolysis, cysteine digestion, tryptophan digestion, and urea. I think I may have it narrowed down but I am hitting some walls. Any ideas? John
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Postby Kammy » Sun Jul 26, 2009 9:45 pm

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Postby mmoir125 » Thu Mar 25, 2010 1:43 am

use an API-20E strip, it should give the must prob
But there's more old drunks than there are old doctors, So I think that I'll have another round!
(Willie Nelson)
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Postby sara135 » Tue Jul 12, 2011 12:00 pm

Through the various tests that were ran in lab, we were able to discern quite a few things about our unknown. The first of which was that it is gram positive, since it was purple after the gram staining. This was supported further by the lack of growth on the EMB plate. It did, however, grow on the MSA plate, which indicates that it can grow in high salt concentrations. Furthermore, it ferments mannitol, since the color of the MSA agar changed. The unknown bacteria was also cocci-shaped and formed clusters.
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Postby jeonkjc » Fri Jul 22, 2011 5:28 pm

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Postby canalon » Fri Jul 22, 2011 6:47 pm

Ifthis will not help if you use something for enterobacteriaceae with a Gram positive...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby becky34 » Mon Aug 01, 2011 8:17 am

Thank you for the link Jeonkjc!!!
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