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autoclave vs pasteurization vs sterilization

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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autoclave vs pasteurization vs sterilization

Postby bbsinfo » Wed Jul 20, 2011 3:01 pm

Hey, I am making a medium: enriched natural seawater medium.
On the receipe, it said pasteurzie, do not autoclave.
I am wondering the differences between pasteurization and autoclaving? And their difference to sterilization?
anyone could help?
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Postby JackBean » Wed Jul 20, 2011 3:11 pm

you don't know the pasteurized milk? Pasteurization is heating for very short time.It should kill bacteria, but not spores and probably also shouldn't destroy some nutrients
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Postby canalon » Wed Jul 20, 2011 7:42 pm

Pasteurization is at lower temperature and short time. Have look at the mighty Wikipedia for the details. It is probably because your medium will form some precipitate if heated at the temperature used for autoclaving. I know Instant Ocean (one brand of artificial seawater) does.
Alternatively you could try microfiltration (that is what I use for Instant Ocean) with a 0.22µm or 0.45µm filter.
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pasteurization v/s sterilization

Postby mjb21 » Fri Jul 22, 2011 1:28 am

hi
Pasteurization is only a method of reducing the microbial load in the given material. it differs from sterilization which is the process of making the material free from all forms of life. Pasteurization aims at killing pathogens and most of the vegetative forms of life. sterility is not assured. After pasteurization your medium will be only "disinfected" but not sterilized. many media are unfavorable for the growth of ordinary contaminants and hence mere pasteurization is sufficient. there is no need to actually sterilize (autoclave) sea water medium as it already poses challenge for the propagation of common micoorganisms. morreover, attempts to sterilize sea water could end up leaving behind cloudy precipitates in the medium..

if you still insist on having a sterile medium only, then you must go in for filtration method of sterilization.
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Postby JorgeLobo » Fri Aug 05, 2011 1:41 pm

Disinfection is an entirely diferent concept versus pasteurization.
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